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Urea (bun) assay

Product Method Size Catalog Price Quantity
Urea (bun) assay UV GLDH 480 Tests UR2821 $194.72
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  • Format
    Mixing and diluting are automatically performed by the DIMENSIONĂ¢ instrument.
  • Assay Range
    0.747 - 61.5mmol/l
  • Working Stability 15-25 °C
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

The BUN method used on the Dimension® clinical chemistry system is an in vitro diagnostic test intended for the quantitative determination of urea nitrogen in serum, plasma and urine. This product is suitable for use on the Siemens Dimension® analyzers.

Clinical Significance

Urea is synthesized in the liver and is a waste product of protein digestion removed from the body in the urine. Urea is measured to monitor patients undergoing dialysis and to evaluate renal and metabolic function.

Higher than normal levels of urea indicate problems with kidney function however levels are also increased as a result of congestive heart failure, heart attack, shock and dehydration. Lower than normal levels are not usually of clinical significance however have been associated with pregnancy and liver disease.


The urea nitrogen method employs a urease/glutamate dehydrogenase coupled enzymatic technique.

Urea is hydrolyzed in the presence of water and urease to produce ammonia and carbon dioxide. The ammonia produced in the first reaction combines with α-oxoglutarate and NADH in the presence of glutamate-dehydrogenase to yield glutamate and NAD+. The change in absorbance at 340 nm due to the disappearance of NADH is directly proportional to the BUN concentration in the sample and is measured using a bichromatic (340, 383nm) rate technique.

Available Applications

  • Dimension dedicated