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Rheumatoid Factor assay

Product Method Size Catalog Price Quantity
Rheumatoid Factor assay Latex Enhanced Immunoturbidimetric R1 2 x 15ml, R2 1 x 10ml RF7980 $502.46
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  • Format
    Liquid ready-to-use
  • Assay Range
    7 - 104IU/ml
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

Latex enhanced immunoturbidimetric test for the quantitative in vitro determination of Rheumatoid Factors (RF) in serum. The test system is intended to be used as an aid in the diagnosis of rheumatoid arthritis. This product is suitable for use on the Hitachi 704, 717, 902, 911 and 912 analysers.

Clinical Significance

The agglutination reaction between antibody coated red blood cells and Rheumatoid Arthritis (RA) sera was first demonstrated by Waaler and Rose in 1940. The reaction has since been shown to be caused by certain factors in the RA sera. These Rheumatoid Factors (RF) are a heterogeneous group of high molecular weight auto antibodies directed against the body’s own immunoglobulins. They are produced by plasma cells present at sites of tissue injury. The initiating antigen is thought to be one or more viruses or viral antigens that persist in the joint tissues. Research has shown that both environmental and genetic factors can affect the production of RF with various biological properties. RF have also been observed in the serum of patients with lupus erythematosus, hepatitis, liver cirrhosis, syphilis and various other conditions but the RF titre is much lower than in RA. Between 60 and 80% of patients with active Rheumatoid Arthritis (RA) possess this abnormal protein in their blood and joint fluid, and its detection is therefore of value in the diagnosis and monitoring of the disease.


Rheumatoid factors are antibodies directed against the Fc portion of IgG. The majority of rheumatoid factors are IgM antibodies, but may be IgG or IgA. Conditions giving rise to such factors include rheumatic conditions and chronic inflammatory processes. The Randox RF latex reagent is a suspension of polystyrene latex particles of uniform size coated with human IgG. When serum containing rheumatoid factor is mixed with the RF latex reagent an increase in turbidity can be measured at 570 nm. By constructing a standard curve from the absorbance of standards, the rheumatoid factor concentration can be determined.

Available Applications

Hitachi 704

Hitachi 717

Hitachi 902

Hitachi 911 / 912