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Lipase assay

Product Method Size Catalog Price Quantity
Lipase assay Colorimetric R1 6 x 20ml, R2 3 x 20ml LI7979 $804.09
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  • Format
    Liquid ready-to-use
  • Assay Range
    3.50 - 651U/l
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Lipase in human serum and plasma. This product is suitable for use on the Hitachi 717.

Clinical Significance

A lipase test system is a device intended to measure the activity of the enzyme lipase in serum and plasma. Lipase measurements are used in the diagnosis and treatment of diseases of the pancreas such as acute pancreatitis and obstruction of the pancreatic duct.

Principle

The chromogenic Lipase substrate 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6ı-methylresorufin) ester is cleaved by the catalytic action of Lipase to form 1,2-o-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6-methyl resorufin) ester. This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin.

The lipase activity in the specimen is proportional to the production of methylresorufin in the reaction and can be determined photometrically.

Available Applications

Hitachi 717