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Lipase assay

Product Method Size Catalog Price Quantity
Lipase assay Colorimetric R1 6 x 20ml, R2 3 x 20ml LI7979 $804.09
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  • Format
    Liquid ready to use
  • Assay Range
    3.5 - 651U/L
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Lipase in human serum and plasma. This product is suitable for use on the Hitachi 717.


Clinical Significance

Lipase is a digestive enzyme released by the pancreas into the small intestine. It is usually measured alongside amylase to help diagnose diseases of the pancreas including acute pancreatitis and obstruction of the pancreatic duct.


Very high levels of lipase in the blood indicate acute pancreatitis; slightly elevated levels are associated with kidney disease or inflammation of the salivary glands.

Individuals suffering from cystic fibrosis, Crohn’s disease and celiac disease may not have enough lipase to the get the nutrition they require from food.


Principle

The chromogenic Lipase substrate 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6?-methylresorufin) ester is cleaved by the catalytic action of Lipase to form 1,2-o-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6-methyl resorufin) ester. This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. The lipase activity in the specimen is proportional to the production of methylresorufin in the reaction and can be determined photometrically.