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CK-NAC assay

Product Method Size Catalog Price Quantity
CK-NAC assay UV DGKC R1 6 x 20ml, R2 3 x 10ml CK7947 $450.30
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  • Format
    Lyophilised
  • Assay Range
    10 - 2000U/L
  • Working Stability 15-25 °C
    3 days
  • Working Stability 2-8 °C
    3 weeks
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Intended Use

For the quantitative in vitro determination of Creatine Kinase in serum or plasma. This product is suitable for use on the Hitachi 704, 705, 717, 902 and 911/912.

Clinical Significance

Creatine Kinase (CK) is primarily found in striated muscle, brain and heart tissues. The determination of CK activity in plasma or serum, provides a sensitive marker for the detection of skeletal muscle disease, e.g. in Duchenne type muscular dystrophy, levels of CK up to 50 times the upper limit of normal may be encountered. In progressive muscular dystrophy, enzyme activity in serum is highest in infancy and childhood.

CK activity is also useful in diagnosis of myocardial infarction and cerebrovascular accidents.

The determination of CK using creatine phosphate and adenosine-5'-diphosphate (ADP) as substrates rather than creatine and adenosine-5'-triphosphate (ATP) has several advantages in test performance as it allows for a faster reaction rate resulting in greater sensitivity. Small sample volumes are used and sample blanks are not required.

Due to the oxidation of the sulphydryl groups in the active site of creatine kinase, it is very unstable. The enzyme however, is reactivated by the addition of thiol compounds.

N-acetyl-L-cysteine (NAC), is included in the test for this reason. Also included in the test system are adenosine-5'- monophosphate (AMP) and diodenosine pentaphosphate, these are present to inhibit myokinase activity which interferes. No sulphates are present in the test system, as these alter the reaction kinetics.

UV Method

This is an optimized standard method according to the recommendations of the Deutsche Gesellschaft für Klinische Chemie.

Available Applications

Hitachi 704

Hitachi 705

Hitachi 717

Hitachi 902

Hitachi 911 / 912