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Bile Acids (5th Gen) (Total) (liquid) assay

Product Method Size Catalog Price Quantity
Bile Acids (5th Gen) (Total) (liquid) assay Enzymatic Colorimetric R1 6 x 50ml, R2 6 x 18ml BI7982 $6125.54
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  • Format
    Liquid ready-to-use
  • Assay Range
    1.47 - 150µmol/l
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Total Bile Acids in serum and plasma. This product is suitable for use on the Hitachi 717, 904, 911 and 912 analysers.

Principle

In the presence of Thio-NAD, the enzyme 3-a hydroxysteroid dehydrogenase (3-a HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-a HSD can convert 3-keto steroids and Thio-NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405nm.

Available Applications

Hitachi 717

Hitachi 904 / 911 / 912