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Bile acids assay

Product Method Size Catalog Price Quantity
Bile acids assay Colorimetric R1 10x10ml, R2 1 x 30ml BI2672 $1950.18
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  • Format
    Lyophilized
  • Assay Range
    Linear up to 150umol/l
  • Working Stability 15-25 °C
    24 hours
  • Working Stability 2-8 °C
    1 week
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Intended Use

For the quantitative in vitro determination of Bile Acids in serum and plasma. This product is suitable for both manual and automated use.


Clinical Significance

Bile Acids tests are used extensively in both human and veterinary medicine to diagnose various liver conditions. Bile Acids are mainly used to diagnose liver conditions such as obstetric cholestasis, portosystemic venous shunts and hepatic microvascular dysplasia however serum levels of total bile acids are also elevated in patients with acute hepatitis, chronic hepatitis, liver sclerosis, and liver cancer. Early detection of liver disease and liver functionality can help patients get effective therapeutic treatment, prevent disease progress, and save lives.

Total Bile Acids are both highly sensitive and specific making them a popular marker of normal liver function.

Bile Acids are of particular importance in birds as elevated levels of enzymes like AST do not always correlate with the presence of liver disease in birds.


Principle


3α-hydroxy bile acids are converted to the corresponding 3-keto bile acids in the presence of NAD+ by 3α-hydroxysteroid dehydrogenase (3α-HSDH). The NADH formed reacts with nitrotetrazolium blue (NBT) in a Δ4DH and Diaphorase catalyzed reaction to form a formazan dye, which has a stable blue color with an absorption maximum at 546 nm. The intensity of the color produced is directly proportional to the bile acids concentration in the sample.


Available Applications

  • Abbott Architect + Aeroset
  • Cobas Mira, Mira S + Mira Plus
  • Dimension
  • Express 550
  • Hitachi 704, 717, 747, 904, 911, 912, 917
  • ILAB 600
  • Konelab 20i, 30i + 60i
  • Olympus AU 400/560/600/ AU2700
  • AU560
  • RA1000/XT/Opera
  • Synchron CX 4, CX 5, CX 7, CX 9, LX20