Home Reagents Clinical Chemistry Reagents Bile Acids (manual) assay
Quality Guranteed Fast Delivery ad for support

Bile Acids (manual) assay

Product Method Size Catalog Price Quantity
Bile Acids (manual) assay Colorimetric R1 10x10ml, R2 1 x 30ml BI2672 $1950.18
Shipping costs will be added at the checkout stage, click here for charges.
  • Format
  • Assay Range
    Linear up to 150umol/l
  • Working Stability 15-25 °C
    24 hours
  • Working Stability 2-8 °C
    1 week
Request Kit Insert

Intended Use

For the quantitative in vitro determination of Bile Acids in serum and plasma. This product is suitable for Manual use.


3-hydroxy bile acids are converted to the corresponding 3-keto bile acids in the presence of NAD+ by 3a-hydroxysteroid dehydrogenase (3a-HSDH). The NADH formed reacts with nitrotetrazolium blue (NBT) in a 4DH and Diaphorase catalyzed reaction to form a formazan dye, which has a stable blue colour with an absorption maximum at 546 nm. The intensity of the colour produced is directly proportional to the bile acids concentration in the sample.