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Bile Acids (manual) assay

Product Method Size Catalog Price Quantity
Bile Acids (manual) assay Colorimetric R1 10x10ml, R2 1 x 30ml BI2672 $1950.18
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  • Format
    Lyophilised
  • Assay Range
    Linear up to 150umol/l
  • Working Stability 15-25 °C
    24 hours
  • Working Stability 2-8 °C
    1 week
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Intended Use

For the quantitative in vitro determination of Bile Acids in serum and plasma. This product is suitable for Manual use.

Principle

3-hydroxy bile acids are converted to the corresponding 3-keto bile acids in the presence of NAD+ by 3a-hydroxysteroid dehydrogenase (3a-HSDH). The NADH formed reacts with nitrotetrazolium blue (NBT) in a 4DH and Diaphorase catalyzed reaction to form a formazan dye, which has a stable blue colour with an absorption maximum at 546 nm. The intensity of the colour produced is directly proportional to the bile acids concentration in the sample.