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Bile acids (liquid) assay

Product Method Size Catalog Price Quantity
Bile acids (liquid) assay Enzymatic Colorimetric R1 2 x 18ml, R2 2 x 8ml BI3863 $822.29
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  • Format
    Liquid ready to use
  • Assay Range
    3.2 - 188µmol/l
  • Working Stability 15-25 °C
    _
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Total Bile Acids in serum and plasma. This product is suitable for use on the RX Series instruments which includes the Rx Daytona and Rx Imola.


Clinical Significance


Bile Acids tests are used extensively in both human and veterinary medicine to diagnose various liver conditions. Bile Acids are mainly used to diagnose liver conditions such as obstetric cholestasis, portosystemic venous shunts and hepatic microvascular dysplasia however serum levels of total bile acids are also elevated in patients with acute hepatitis, chronic hepatitis, liver sclerosis, and liver cancer. Early detection of liver disease and liver functionality can help patients get effective therapeutic treatment, prevent disease progress, and save lives.

Total Bile Acids are both highly sensitive and specific making them a popular marker of normal liver function.

Bile Acids are of particular importance in birds as elevated levels of enzymes like AST do not always correlate with the presence of liver disease in birds.


Principle


In the presence of Thio-NAD, the enzyme 3-α hydroxysteroid dehydrogenase (3-α HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-α HSD can convert 3-keto steroids and Thio-NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405nm.


Available Applications

  • RX daytona, RX imola, RX monza
  • Abbott Architect + Aeroset
  • Alcyon 300 + Falcor
  • BT2000/BT3000/ILAB300/TARGA
  • Cobas 6000, Cobas Fara
  • Cobas Mira, Mira S + Mira Plus
  • Express 550
  • Hitachi 717, 904, 911, 912, 917
  • ILAB 300 Plus, 600
  • Integra 400, 800
  • Konelab 20i, 30i + 60i
  • Olympus AU 400/560/600/ AU2700
  • Ortho Fusion
  • Pentra 400
  • Prestige
  • RA1000/XT/Opera
  • Synchron CX 4, CX 5, CX 7, CX 9, LX20
  • Vitalab Flexor, Selectra E + Selectra II
  • Dimension