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Asparate Aminotransferase (AST) assay (manual)

Product Method Size Catalog Price Quantity
Asparate Aminotransferase (AST) assay (manual) Colorimetric 2 x 100ml AS147 $70.92
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  • Format
    Liquid ready-to-use
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Aspartate Aminotransferase (AST) in serum. This product is suitable for Manual use.

Clinical Significance

Clinical Significance

The aminotransferases are a group of enzymes that catalyse the inter conversions of amino acids and -oxoacids by transfer of amino groups. AST (aspartate aminotransferase or glutamate oxaloacetate transaminase) has been found in the cytoplasm and the mitochondria of cells that have been studied. In cases of mild tissue damage, e.g. liver, the predominant form of serum AST is that from the cytoplasm, with a smaller amount coming from the mitochondria. Severe tissue damage will result in more mitochondrial enzyme being released. Elevated levels of AST can signal myocardial infarction, hepatic disease, muscular dystrophy and organ damage.

Although heart muscle is found to have the most activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue and kidneys of humans. The IFCC has now recommended (1980) standardised procedures for AST determinations including:-

1. optimization of substrate concentrations.

2. Employment of Tris buffers (instead of phosphate, which has been shown to inhibit recombination of the apoenzyme with pyridoxal phosphate).

3. Pre-incubation of combined buffer and serum to allow side reactions with NADH to occur.

4. Substrate start (-oxoglutarate)

5. Optional pyridoxal phosphate activation. This is an optimised standard method according to the recommendations of the IFCC.

Colorimetric Method

Colorimeteric method (Reitman and Frankel) for determination of serum aspartate aminotransferase.

Principle

Aspartate Aminotransferase is measured by monitoring the concentration of oxaloacetate hydrazone formed with 2,4-dinitrophenyl-hydrazine.

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