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Asparate Aminotransferase (AST) assay (manual)

Product Method Size Catalog Price Quantity
Asparate Aminotransferase (AST) assay (manual) Colorimetric R1 1 x 100ml, R2 1 x 100ml AS101 $50.98
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  • Format
    Liquid ready-to-use
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of Aspartate Aminotransferase (AST) in serum. This product is suitable for Manual use.

Clinical Significance

The aminotransferases are a group of enzymes that catalyze the inter conversions of amino acids and a­oxoacids by transfer of amino groups. AST (aspartate aminotransferase or glutamate oxaloacetate transaminase) has been found in the cytoplasm and the mitochondria of cells that have been studied. In cases of mild tissue damage e.g. liver the predominant form of serum AST is that from the cytoplasm, with a smaller amount coming from the mitochondria.

Severe tissue damage will result in more mitochondrial enzyme being released.

AST is usually tested alongside ALT to diagnose liver damage. AST levels are sometimes compared directly to ALT and an AST/ALT ratio is calculated. This ratio may be used to distinguish between different causes of liver damage.

Extremely high levels of AST are associated with acute hepatitis and are usually due to viral infection. Levels can also be high as a result of drugs or other substances toxic to the liver. Levels are moderately high in chronic hepatitis or cirrhosis.

Elevated levels of AST can also signal myocardial infarction, muscular dystrophy and organ damage. Although heart muscle is found to have the most activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue and kidneys of humans.

Principle

Colorimetric method (Reitman and Frankel) for determination of serum aspartate aminotransferase.

AST is measured by monitoring the concentration of oxaloacetate hydrazone formed with 2,4-dinitrophenylhydrazine.

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