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Asparate Aminotransferase (AST) (liquid) assay

Product Method Size Catalog Price
Asparate Aminotransferase (AST) (liquid) assay Modified IFCC R1 6x68, R2 6x20 AS8005 POA
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  • Format
    Liquid ready-to-use
  • Assay Range
    7.70U/l - 1111U/l
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

An AST test system is a device intended for the quantitative in vitro determination of aspartate aminotransferase (AST) activity in serum and plasma. This product is suitable for use on RX series analyser RX modena.

Clinical Significance

The aminotransferases are a group of enzymes that catalyse the inter conversions of amino acids and -oxoacids by transfer of amino groups. AST (aspartate aminotransferase or glutamate oxaloacetate transaminase) has been found in the cytoplasm and the mitochondria of cells that have been studied. In cases of mild tissue damage (for example, liver) the predominant form of serum AST is that from the cytoplasm, with a smaller amount coming from the mitochondria. Severe tissue damage will result in more mitochondrial enzyme being released. Elevated levels of AST can signal myocardial infarction, hepatic disease, muscular dystrophy and organ damage.

Although heart muscle is found to have the most activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue and kidneys of humans. The IFCC has now recommended (1980)1 standardised procedures for AST determinations including:-

1. Optimisation of substrate concentrations.

2. Employment of Tris buffers (instead of phosphate, which has been shown to inhibit recombination of the apoenzyme with pyridoxal phosphate).

3. Pre-incubation of combined buffer and serum to allow side reactions with NADH to occur.

4. Substrate start (-oxoglutarate).

5. Optional pyridoxal phosphate activation. This is an optimised standard method according to the recommendations of the IFCC.

UV Method

This is a modification of the optimised standard method according to the recommendations of the IFCC.

Principle

a-oxoglutarate reacts with L-aspartate in the presence of AST to form L-glutamate plus oxaloacetate. The indicator reaction utilises the oxaloacetate for a kinetic determination of NADH consumption.

Available Applications

RX Modena