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Apolipoprotein B (Apo B) assay

Product Method Size Catalog Price Quantity
Apolipoprotein B (Apo B) assay Immunoturbidimetric R1 4 x 50ml, R2 4 x 9ml LP2117 $1318.30
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  • Format
    Liquid ready-to-use
  • Assay Range
    11.5 - 219mg/dl
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

For the quantitative in vitro determination of apolipoprotein B (apo B) in serum and plasma. This product is suitable for use on Hitachi 704/717/902/911/912.

Clinical Significance

Lipids are synthesised in the intestine or liver but must be transported to tissues and organs. However this is not possible without hydrophilic adaptation. Lipids are therefore transported by a series of micellar structures. These structures consist of an outer monolayer of protein (an apolipoprotein) and polar lipids (phospholipids and unesterified cholesterol) plus an inner core of neutral lipids (triglycerides and cholesterol esters). The apolipoproteins interact with a series of enzymes and tissue receptors and are therefore responsible for further metabolism and catabolism of the micelle.

The B apolipoproteins are the main form of protein found in low density lipoproteins (LDL). Two forms of apo B are found in humans. The most common form is B-100 (or large B) which constitutes the apo B found in lipoproteins synthesised in the liver. The other form is B-48 (or small B), thought to be synthesised in the intestinal wall. Apo B is the main cholesterol carrying protein in the blood and is the ligand concerned with the uptake of cholesterol into cells by the LDL - receptor pathway. Apo B shows atherogenic signs and is thus useful for the evalution of coronary risk.

Studies have shown that there is an inverse relationship between APO A-1 and coronary artery disease and a direct relationship with APO B such that pateints with CAD have generally reduced levels of APO A-1 and increased levels of APO B.


This method is based on the reaction of a sample containing human apo B and a specific antiserum to form an insoluble complex which can be measured turbidimetrically at 340 nm. By constructing a standard curve from the absorbances of standards the concentration of apo B can be determined.

Available Applications

Hitachi 704

Hitachi 717

Hitachi 902

Hitachi 911 / 912