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Amylase (pancreatic) (liquid) assay

Product Method Size Catalog Price Quantity
Amylase (pancreatic) (liquid) assay Ethylidene Blocked-pNPG7 R1 4 x 16ml, R2 4 x 5ml AY3855 $767.81
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  • Format
    Liquid ready-to-use
  • Assay Range
    9 - 1157 U/L
  • Working Stability 2-8 °C
    Stable to expiry
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Intended Use

A Pancreatic a-Amylase test system is a device intended for the quantitative in vitro determination of Pancreatic a-Amylase activity in serum, plasma and urine. This product is suitable for use on RX series instruments which includes the RX daytona and the RX imola.

Clinical Significance

The amylases are a group of hydrolases that split complex carbohydrates constituted of -D-glucose units. The two recognised types of amylases are ß-amylase (e.g. plant and bacterial exoamylase) and human -amylases which can attack the a-1, 4-linkage anywhere along a polyglucan chain.

Human a-amylase consists of two major isoenzymes, pancreatic and salivary, which are encoded by two different genes. Pancreatic amylase is synthesised only in pancreatic tissue by acinar cells. Amylase measurements are used primarily for the diagnosis and treatment of pancreatitis (inflammation of the pancreas). The evaluation of the pancreatic isoamylase may have a greater clinical specificity for the diagnosis of pancreatic disorders than total amylase assessment.

Principle

Two monoclonal antibodies are incubated with the sample to inhibit the salivary amylase present but do not affect pancreatic amylase. This method uses ethylidene-p-nitrophenyl maltoheptaoside as the substrate. The substrate is then added and any amylase present splits the substrate to produce oligosaccharides and pNP-G2, pNP-G3 and pNP-G4.

a-glucosidase is added as the indicator enzyme to release the p-nitrophenol (p-NP). The final result of the hydrolysis by amylase and -glucosidase is free p-NP, which is detected by its absorbance at 405 nm. The terminal glucose is blocked preventing cleavage by the indicator enzyme.

Available Applications

RX series instruments, including:

RX daytona

RX imola